To find compounds like small molecules, peptides, or RNAi that change the phenotype of a cell or an organism in a desirable way, phenotypic screening is a sort of screening used in biological research and drug development. To understand how a phenotypic hit functions, phenotypic screening must be followed by identification and confirmation, frequently using chemoproteomics. Historically, medication discovery has been based on phenotypic screening. Compounds are tested in cellular or animal illness models to find those that result in a desired change in phenotype. The procedure of target deconvolution is used to identify the biological targets of chemicals only after they have been found. This comprehensive plan is known as target deconvolution. This overall strategy is referred to as "classical pharmacology", "forward pharmacology" or "phenotypic drug discovery" (PDD).
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