Long noncoding RNA FGD5-as1 sponges microRNA-497-5p to regulate hyperuricaemia-induced renal interstitial fibrosis in a rat model

Objectives: To explore the roles of lncRNA FGD5 antisense RNA 1 (FGD5-AS1) and miR-497-5p in Renal Interstitial Fibrosis (RIF).

Methods: Rat hyperuricaemia models were constructed and respectively treated with altered FGD5 AS1 or miR-497-5p to detect biochemical indices including Uric Acid (UA), Serum Creatine (SCr), Blood Urea Nitrogen (BUN) and 24-h urine protein. The pathological changes and score, fibrosis degree and RIF index in rat kidney were determined. Expression of FGD5-AS1, miR-497-5p, LIM Domain Only 7 (LMO7), ZO-1 and Occludin was assessed.

Results: In kidney tissues from hyperuricaemia rats, FGD5-AS1 and LMO7 were downregulated while miR-497-5p was upregulated. Overexpressed FGD5-AS1 or reduced miR-497-5p reversed RIF-induced changes in hyperuricaemia rats, while downregulated FGD5-AS1 or upregulated miR-497-5p had opposite effects.

Conclusions: Overexpressed FGD5-AS1 downregulated miR-497-5p to ameliorate RIF in hyperuricaemia rats by promoting LMO7.