Title : Comparative study on the resistance of beta-cypermethrin nanoemulsion and conventional emulsion in Blattella germanica
Abstract:
Objective: This study aimed to compare the resistance rates of Blattella germanica to beta-cypermethrin nanoemulsion and conventional emulsion and establish reference values via biochemical detection for conventional emulsion.
Methods: We conducted experiments using subcultured Blattella germanica and applied the micro-drop method for treatment. Subsequently, the activity of metabolic enzymes was measured using spectrophotometry. Profile analysis was employed to study the resistance rates of beta-cypermethrin nanoemulsion and beta-cypermethrin emulsion.
Results: The regression equation for the relationship between generation and resistance factor in Blattella germanica treated with beta-cypermethrin nanoemulsion was as follows: y1 = 0.091x1 + 0.991, with an r-value of 0.990 (F = 95.184, p = 0.01 < 0.05). Similarly, the regression equation for Blattella germanica treated with emulsion was y2 = 0.376x2 + 1.051, with an r-value of 0.993 (F = 141.094, p = 0.007 < 0.05). The comparison of slopes between these two regression equations yielded an F-value of 8.61, indicating a significant difference (p = 0.001 < 0.05).
Conclusion: Our findings suggest that the resistance factor in Blattella germanica treated with beta-cypermethrin nanoemulsion differs from that treated with beta-cypermethrin emulsion. Specifically, the resistance factor of beta-cypermethrin nanoemulsion increased at a slower rate compared to beta-cypermethrin emulsion.
Audience Take Away:
When presenting your research titled "Comparative Study on the Resistance of Beta-Cypermethrin Nanoemulsion and Conventional Emulsion in Blattella germanica," the audience will learn the following specific items:
- The audience will gain an understanding of the resistance rates of Blattella germanica to beta-cypermethrin nanoemulsion versus conventional emulsion, enhancing their knowledge of insect resistance.
- You will explain the methods used to establish reference values for conventional emulsion through biochemical detection, providing a practical guide for evaluating insect resistance to chemicals.
- By sharing experimental procedures such as using subcultured Blattella germanica and the micro-drop method of treatment, as well as measuring metabolic enzyme activity using spectrophotometry, you will help the audience grasp the experimental design and techniques involved.
These points demonstrate the potential impact of your research on the audience, including offering new insights into insect resistance, guiding the assessment of chemical impacts on insects, and providing valuable research experience for other scholars to expand their own work or teaching. This knowledge will assist the audience in gaining a deeper understanding of insect resistance mechanisms, enabling them to make more informed decisions when designing effective pest control measures, thereby improving their job efficiency and the accuracy of their outcomes.
