Title : Evaluation of SIRT1 as a potential biomarker of aging among middle-aged individuals with altered serum vitamin d status
Abstract:
Background: The role of Vitamin D in promoting overall cellular health and preventing age-related diseases is well established. Further, studies have reported that the Sirtuin1 gene regulates aging, promotes longevity, and is recognized as one of the molecular hallmarks of biological aging.
Objectives: The study's primary objective is to compare the serum sirtuin 1 level among individuals with and without vitamin D deficiency. The secondary objective is to evaluate the association of SIRT1 gene polymorphism with vitamin D levels.
Methods: A total of 174 subjects were recruited, including 87 subjects with vitamin D deficiency and 87 with normal vitamin D levels. Serum vitamin D and sirtuin 1 levels were measured by enzyme-linked immunosorbent assay (ELISA). Genotyping was performed with the blood sample for Single Nucleotide Polymorphism (SNP)-rs3740051 of the SIRT1 gene by using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP). A comparison of variables between the two groups was performed using the Mann-Whitney U test. Spearman’s correlation test assessed the correlation between sirtuin 1 and vitamin D. The association between SIRT1 gene polymorphism and vitamin D levels was analyzed by chi-square test. Hardy-Weinberg equilibrium was calculated for the alleles.
Conclusion: The findings of the study show an evident association between vitamin D deficiency and higher SIRT1 gene polymorphism and the down expression of SIRT1 among middle-aged adults. Therefore, the study concludes that Individuals with vitamin D deficiency exhibit altered SERT1 gene expression mediated accelerated biological aging. Further, maintaining optimal vitamin D levels through lifestyle modifications, dietary interventions, or appropriate pharmacological strategies is essential for mitigating premature aging.
